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Role of coagulase-negative staphylococci in color development of fermented meat products based on nitric oxide
Risu Na1, Jiawang Wang1, Jiasheng Lu1
1College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
Abstract:
This study investigated whether nitroso-myoglobin (NO-Mb) can form in a nitrite-free simulated system via nitric oxide synthase (NOS) activity from three coagulase-negative staphylococci (CNS): Staphylococcus saprophyticus HRB1, S. saprophyticus HRB2, and Mammaliicoccus vitulinus HRB1. Treatments inoculated with these CNS showed significantly higher redness (a*-value) and UV-Visible absorbance than the negative control (Pediococcus pentosaceus). All three strains exhibited measurable NOS activity (0.23, 0.19, and 0.21 U/mL, respectively), and inhibitor assays further implicated NOS activity in these strains. PCR analysis confirmed expression of the nos gene, and confocal laser scanning microscopy detected NO generation in each strain. Pentacoordinate NO-Mb formed in both broth and meat batter upon inoculation with any of the CNS strains, with S. saprophyticus HRB1 yielding the highest NO-Mb concentration. These findings suggest a viable route for producing nitrite-free fermented meats using health-conscious formulations.
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