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Published on: December 15, 2017
Metabolic engineering tools and strategies for Corynebacterium glutamicum
Cheon Woo Moon1, Sumin Cho1, Minjung Ki1
1Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 FOUR Program), Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea; BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
Trends in Biotechnology
|June 10, 2026
Summary
Corynebacterium glutamicum is a versatile microbial factory for producing amino acids and chemicals. This review details genetic tools and metabolic engineering strategies to optimize its biomanufacturing capabilities.
Area of Science:
- Microbiology
- Synthetic Biology
- Biotechnology
Background:
- Corynebacterium glutamicum is a key industrial microorganism.
- Traditionally used for amino acid production.
- Expanding use as a chassis for diverse chemical synthesis.
Purpose of the Study:
- To review genetic engineering tools for C. glutamicum.
- To discuss metabolic engineering strategies for chemical production.
- To outline future directions for biomanufacturing.
Main Methods:
- Summarizing genetic engineering tools.
- Discussing metabolic engineering strategies: flux optimization, enzyme/transporter engineering, dynamic regulation, adaptive laboratory evolution.
- Reviewing systems-level approaches.
Main Results:
- Comprehensive overview of C. glutamicum genetic tools.
- Detailed discussion of metabolic engineering techniques for enhanced chemical yields.
- Identification of challenges and future prospects.
Conclusions:
- C. glutamicum holds significant potential for sustainable biomanufacturing.
- Advanced tools and strategies are crucial for its optimization.
- Further research is needed to overcome current limitations.
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