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Published on: December 15, 2017
Metabolic engineering tools and strategies for Corynebacterium glutamicum
Cheon Woo Moon1, Sumin Cho1, Minjung Ki1
1Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 FOUR Program), Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea; BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
Abstract:
Corynebacterium glutamicum is a prominent industrial microorganism traditionally employed for amino acid production, and its utility has been progressively expanded as a chassis for producing diverse valuable chemicals. To fully exploit this potential, advanced synthetic biology toolkits and metabolic engineering strategies have been rapidly developed. In this review, we first summarize widely utilized and recently developed tools for genetic engineering in C. glutamicum. We then discuss key metabolic engineering strategies to enhance the production of target chemicals, with an emphasis on systems-level flux optimization, enzyme engineering, transporter engineering, dynamic regulation, and adaptive laboratory evolution. Finally, we outline current challenges and future directions for advancing C. glutamicum as a competitive platform for sustainable biomanufacturing.
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