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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: Jun 12, 2026

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
09:30

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

Published on: June 2, 2022

On-chip patterned light source that superimposes multiple patterns for speckle reduction.

Yoshitaka Kurosaka, Yutaka Takagi, Akio Ito

    Optics Express
    |June 11, 2026
    PubMed
    Summary
    This summary is machine-generated.

    Researchers created a novel on-chip patterned light source. This device successfully superimposes four beam patterns with reduced speckle contrast, advancing optical technology.

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    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

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    Last Updated: Jun 12, 2026

    Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
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    Published on: June 2, 2022

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    Published on: March 2, 2011

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    Area of Science:

    • Optics and Photonics
    • Microfabrication

    Background:

    • Developing integrated optical systems requires precise control over light emission.
    • On-chip light sources are crucial for miniaturized optical devices.

    Purpose of the Study:

    • To design and demonstrate an on-chip patterned light source capable of superimposing multiple beam patterns.
    • To evaluate the performance and image quality of the superimposed beams.

    Main Methods:

    • Fabrication of an 800 micrometer square chip with four elementary pattern areas.
    • Emission pattern analysis in the far-field region for individual and combined elementary pattern areas.
    • Comparison of far-field patterns to assess superposition quality and speckle contrast.

    Main Results:

    • Successful superposition of four elemental images from each elementary pattern area without blurring.
    • Demonstration of a unique center axis emission for all pattern areas.
    • Significant reduction in speckle contrast achieved in the superimposed pattern.

    Conclusions:

    • The developed on-chip patterned light source effectively superimposes multiple beam patterns.
    • The device shows potential for applications requiring high-quality, superimposed light fields with reduced speckle.
    • The compact design and successful performance highlight advancements in integrated optics.