Protocol for direct in vivo evaluation of the stretch reflex in adult mice

Sensory feedback from muscle afferents that recruit spinal pathways is essential for coordination and stability. Dysregulation of these neural circuits may result in impaired coordinated motor behavior. Here, we present a protocol for assessing the stretch reflex in the adult decerebrate mouse. We describe steps for leg dissection, recording setup, decerebration, and reflex recording. This protocol can capture changes in muscle mechanics, sensory encoding, and ultimately muscle force generation under varying conditions. For complete details on the use and execution of this protocol, please refer to Rotterman et al.¹.