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Related Experiment Video

Updated: Jun 13, 2026

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
06:15

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Published on: August 18, 2018

Measuring efferocytosis by intraperitoneal clearance assay.

Caitlin L Vella1, Dilara C Ozkocak2, Amy A Baxter1

  • 1La Trobe Institute for Molecular Science, Department of Biochemistry & Chemistry, School of Agriculture, Biomedicine and Environment, La Trobe University, Bundoora, VIC, Australia; School of Agriculture, Biomedicine and Environment, Centre for Cardiovascular Biology and Disease Research, La Trobe University, Bundoora, VIC, Australia.

Methods in Cell Biology
|June 11, 2026
PubMed
Summary

This study introduces an in vivo method to measure efferocytosis, the process of clearing apoptotic cells. The intraperitoneal clearance assay in mice offers a more accurate assessment of this vital immune function in a physiological context.

Keywords:
ApoptosisEfferocytosisFlow cytometryIntraperitioneal clearancePhagocytes

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Area of Science:

  • Immunology
  • Cell Biology
  • Physiology

Background:

  • Efferocytosis, the clearance of apoptotic cells by phagocytes, is crucial for immune tolerance and tissue homeostasis.
  • In vitro assays for efferocytosis lack the complexity of the in vivo physiological environment.
  • A need exists for robust, in vivo methods to study efferocytosis.

Purpose of the Study:

  • To present a detailed in vivo approach for assessing efferocytosis by mouse peritoneal macrophages.
  • To enable quantification of apoptotic cell uptake in a native tissue context.
  • To provide a framework for investigating interventions affecting efferocytic capacity.

Main Methods:

  • Utilized an intraperitoneal (IP) clearance assay in mice.
  • Administered fluorescently labeled apoptotic Jurkat T cells into the peritoneal cavity.
  • Quantified apoptotic cell uptake by peritoneal macrophages in vivo.

Main Results:

  • The IP clearance assay allows for the quantification of efferocytosis in a native tissue environment.
  • This method captures the influence of local immune signals, stromal interactions, and systemic factors.
  • The protocol is adaptable for genetic and pharmacological manipulations.

Conclusions:

  • The described in vivo approach provides a physiologically relevant framework for studying efferocytosis.
  • This method enhances understanding of efferocytosis in both homeostatic and pathological settings.
  • It facilitates research into the regulation and dysfunction of apoptotic cell clearance.