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Updated: Jun 13, 2026

Cell-Free DNA Extraction of Vitreous and Aqueous Humor Specimens for Diagnosis and Monitoring of Vitreoretinal Lymphoma
Published on: January 12, 2024
Vitreoretinal Lymphoma: a clinical case highlighting the diagnostic and therapeutic challenge and the need for a
María Sanchidrián Mayo1, Almudena Cabero Martínez2, Luis Cacharro Moras3
1Ophtalmology Department, University Hospital of Salamanca, Salamanca, Spain. msanchidrianma@saludcastillayleon.es.
Abstract:
Vitreoretinal Lymphoma (VRL) is a rare ocular malignancy often presenting with atypical retinal findings, which can lead to misdiagnosis. We report the case of a patient with bilateral intraocular lesions and reduced visual acuity. Diagnosis was confirmed by multimodal imaging and molecular analysis of aqueous humor. Treatment consisted of intravitreal methotrexate and rituximab, followed by systemic chemotherapy, achieving partial resolution of the ocular lesions but subsequent ocular and systemic recurrence. After rescue treatment, patient achieved sustained complete remission. Early diagnosis and a multidisciplinary approach are essential, and multimodal imaging combined with molecular diagnostics is key in confirming the diagnosis.
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