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Updated: Jun 13, 2026

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
Published on: August 20, 2012
Preparation and Photophysical Study of Rhodamine-Perylenebisimide Electron Donor-Acceptor Dyad/Triads Containing
Xin Guan1, Haotian Bai2, Jianzhang Zhao1
1State Key Laboratory of Fine Chemicals, Frontiers Science Center for Smart Materials Oriented Chemical Engineering, School of Chemical Engineering, Dalian University of Technology, 2 Ling Gong Road, Dalian 116024, China.
Abstract:
We report the synthesis and characterization of the photophysical characterization of a series of rhodamine (Rho)-perylenebisimide (PBI) electron donor-acceptor dyad/triads containing flexible alkyl spacers (ethylene or hexylene chains). Steady-state absorption and emission, femtosecond and nanosecond transient absorption (fs-TA and ns-TA), cyclic voltammetry, triplet-triplet energy transfer (TTET) experiments and DFT/TD-DFT calculations were combined to elucidate the excited-state dynamics. fs-TA spectral study indicates fast decay of the S1 state and formation of the 3PBI state (0.32-663 ps), which is supported by the ns-TA spectra. The localized PBI triplet (3PBI*) exhibits unusually long lifetimes (up to 272 μs) as determined by the TTET experiment. No long-lived charge-separated (CS) state was observed. While a Förster resonance energy transfer (FRET) probably occurs between PBI and the open-ring rhodamine, a photo-induced electron transfer is proposed to be responsible for the quenching of the fluorescence of the PBI moiety.
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