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Published on: July 27, 2022

OV16 Improves Radiation-Induced Intestinal Injury by Targeting Transglutaminase 2.

Zhiyan Zhang1,2, He Wang2,3, Yaowen Cui2

  • 1College of Life Sciences, Hebei University, Baoding 071002, China.

Molecules (Basel, Switzerland)
|June 12, 2026
PubMed
Summary
This summary is machine-generated.

Orychophragine D (OV16), a natural alkaloid, protects against radiation-induced intestinal injury (RIII). It targets transglutaminase 2 (TGM2), enhancing cell repair and reducing damage, offering a new therapeutic strategy.

Keywords:
OV16Orychophragmus violaceusTransglutaminase 2 (TGM2)radiation-induced intestinal injury (RIII)

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Area of Science:

  • Gastroenterology
  • Radiation Oncology
  • Pharmacology

Background:

  • Radiation-induced intestinal injury (RIII) involves cell death, stem cell damage, and barrier dysfunction.
  • Natural small molecules offer potential therapeutic avenues for RIII.

Purpose of the Study:

  • To investigate the radiation protection effect of Orychophragine D (OV16) on RIII.
  • To elucidate the molecular mechanism underlying OV16's protective effects.

Main Methods:

  • In vitro studies using irradiated human small intestinal epithelial cells (HIEC-6).
  • Identification of OV16's cellular target using DARTS, molecular docking, MD simulation, CETSA, and MST.
  • Assessment of TGM2's role in mitigating radiation-induced damage.

Main Results:

  • OV16 demonstrated significant protection against radiation in HIEC-6 cells.
  • Transglutaminase 2 (TGM2) was identified as the key cellular target of OV16.
  • OV16 upregulated nuclear TGM2 expression, enhancing cell proliferation and migration while reducing DNA damage (γ-H2AX).

Conclusions:

  • TGM2 is a novel therapeutic target for RIII.
  • OV16 serves as a molecular template for developing TGM2 allosteric activators for RIII treatment.