Related Experiment Video
Updated: Jun 13, 2026
09:10
Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
Published on: July 27, 2022
OV16 Improves Radiation-Induced Intestinal Injury by Targeting Transglutaminase 2
Zhiyan Zhang1,2, He Wang2,3, Yaowen Cui2
1College of Life Sciences, Hebei University, Baoding 071002, China.
Molecules (Basel, Switzerland)
|June 12, 2026
Summary
Orychophragine D (OV16), a natural alkaloid, protects against radiation-induced intestinal injury (RIII). It targets transglutaminase 2 (TGM2), enhancing cell repair and reducing damage, offering a new therapeutic strategy.
Area of Science:
- Gastroenterology
- Radiation Oncology
- Pharmacology
Background:
- Radiation-induced intestinal injury (RIII) involves cell death, stem cell damage, and barrier dysfunction.
- Natural small molecules offer potential therapeutic avenues for RIII.
Purpose of the Study:
- To investigate the radiation protection effect of Orychophragine D (OV16) on RIII.
- To elucidate the molecular mechanism underlying OV16's protective effects.
Main Methods:
- In vitro studies using irradiated human small intestinal epithelial cells (HIEC-6).
- Identification of OV16's cellular target using DARTS, molecular docking, MD simulation, CETSA, and MST.
- Assessment of TGM2's role in mitigating radiation-induced damage.
Main Results:
- OV16 demonstrated significant protection against radiation in HIEC-6 cells.
- Transglutaminase 2 (TGM2) was identified as the key cellular target of OV16.
- OV16 upregulated nuclear TGM2 expression, enhancing cell proliferation and migration while reducing DNA damage (γ-H2AX).
Conclusions:
- TGM2 is a novel therapeutic target for RIII.
- OV16 serves as a molecular template for developing TGM2 allosteric activators for RIII treatment.
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