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A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes
Published on: February 17, 2023
Ginkgo biloba Flower Extract Accelerates the Wound Healing in Diabetic Rats by Inhibiting Inflammation and
Xin Sun1,2, Ruihong Li2, Yingying Xu1,2
1School of Pharmacy, Anhui Medical University, Hefei 230032, China.
International Journal of Molecular Sciences
|July 15, 2026
Summary
Ginkgo biloba flower extract (GBF) accelerates diabetic wound healing by reducing inflammation and ferroptosis. This natural extract promotes tissue repair and blood vessel formation in diabetic wound models.
Area of Science:
- Biomedical Science
- Pharmacology
- Wound Healing Research
Background:
- Diabetic wounds are a significant clinical challenge, often complicated by impaired healing due to ferroptosis and persistent inflammation.
- Ferroptosis, a form of regulated cell death, and chronic inflammation exacerbate the poor healing outcomes in diabetic patients.
Purpose of the Study:
- To investigate the therapeutic potential of Ginkgo biloba flower extract (GBF) in promoting diabetic wound healing.
- To elucidate the underlying mechanisms of GBF action, focusing on its effects on inflammation and ferroptosis.
Main Methods:
- Chemical composition analysis of GBF identified key active compounds, including flavonoids and terpenoids.
- In vivo studies utilized diabetic rat models to assess GBF's impact on wound closure, epithelial regeneration, collagen deposition, and angiogenesis (CD31, VEGF expression).
- In vitro experiments examined GBF's effects on rat skin fibroblasts (RS1) and its ability to mitigate ferroptosis markers (ROS, Fe2+) in erastin-induced cells. Proteomics analysis was employed to confirm pathway regulation.
Main Results:
- GBF treatment significantly accelerated wound healing in diabetic rats, enhancing epithelial regeneration and collagen deposition.
- GBF promoted new blood vessel formation by increasing CD31 and VEGF expression.
- Mechanistically, GBF inhibited inflammation by reducing TNF-α and IL-6 levels, and suppressed ferroptosis by increasing GSH and GPX4 levels. In vitro, GBF improved fibroblast survival and migration while reducing ROS and Fe2+ in ferroptosis models.
Conclusions:
- Ginkgo biloba flower extract (GBF) demonstrates significant efficacy in promoting diabetic wound healing.
- GBF exerts its therapeutic effects by concurrently inhibiting ferroptosis and suppressing inflammatory responses.
- The findings suggest GBF as a promising natural therapeutic agent for managing diabetic wounds.