Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cadmium induced oxalic acid secretion and its role in metal uptake and detoxification mechanisms in Phanerochaete chrysosporium.

Applied microbiology and biotechnology·2014
Same author

[AS-PCR assay for 20 mtDNA SNP typing and haplotype frequency].

Fa yi xue za zhi·2014
Same author

Identification of ANKK1 rs1800497 variant in schizophrenia: new data and meta-analysis.

American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics·2014
Same author

Predictors of consistent condom use among Chinese female sex workers: an application of the protection motivation theory.

Health care for women international·2014
Same author

Overexpression of PEP-19 suppresses angiotensin II-induced cardiomyocyte hypertrophy.

Journal of pharmacological sciences·2014
Same author

Altered brain network modules induce helplessness in major depressive disorder.

Journal of affective disorders·2014
Same journal

Mechanical forces of beating heart suppress cancer growth.

Mechanobiology in medicine·2026
Same journal

Mechanobiology of hypertrophic chondrocyte mineralization: Biomechanical regulation and therapeutic implications in skeletal disorders.

Mechanobiology in medicine·2026
Same journal

Mechanochemical bistability as a principle of robust morphogenesis.

Mechanobiology in medicine·2026
Same journal

Mechanosensitive reactive oxygen Species:A precision medicine target for oxidative stress-related diseases.

Mechanobiology in medicine·2026
Same journal

Viscous drag-induced self-regeneration of laser-dissected actin stress fibers: A potential mechanism for mechanical memory.

Mechanobiology in medicine·2026
Same journal

Numerical analysis of three-dimensional middle ear and inner ear based on nonlinear and viscoelastic constitutive relations.

Mechanobiology in medicine·2026
See all related articles

Related Experiment Video

Updated: Jun 13, 2026

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
07:45

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

Substrate pre-stretch reprograms macrophage behavior through surface topographical remodeling.

Gang Yang1, Yi Han2, Tao Tang3

  • 1Department of General Surgery, Affiliated Hospital of North Sichuan Medical College, National Clinical Key Specialty (General Surgery), Sichuan Branch of National Clinical Research Center for Digestive Diseases, Sichuan Clinical Research Center for Digestive Diseases, No. 63 Wenhua Road, Nanchong, 637000, PR China.

Mechanobiology in Medicine
|June 12, 2026
PubMed
Summary
This summary is machine-generated.

Mechanically preconditioned surfaces instruct macrophage behavior, altering cell shape, migration, and promoting a pro-inflammatory response. This reveals substrate mechanics as a key regulator in macrophage mechanoimmunology.

Keywords:
Cell adhesionCell migrationCell morphologyImmune responseMacrophagePre-stretch

More Related Videos

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays
08:37

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Published on: April 18, 2025

In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages
12:27

In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages

Published on: December 30, 2017

Related Experiment Videos

Last Updated: Jun 13, 2026

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
07:45

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays
08:37

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Published on: April 18, 2025

In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages
12:27

In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages

Published on: December 30, 2017

Area of Science:

  • Biomaterials Science
  • Immunology
  • Cell Biology

Background:

  • Mechanical forces significantly influence cell behavior, but the long-term biological effects of preconditioned surfaces are not fully understood.
  • Macrophages are critical immune cells whose functions are sensitive to their microenvironment.

Purpose of the Study:

  • To investigate if mechanically preconditioned polydimethylsiloxane (PDMS) surfaces can continuously direct macrophage behavior after mechanical loading ceases.
  • To explore the impact of substrate mechanical properties on macrophage morphology, adhesion, migration, and inflammatory polarization.

Main Methods:

  • Developed a uniaxial pre-stretch device to create PDMS membranes with defined tensile histories.
  • Utilized atomic force microscopy to characterize surface topography changes.
  • Cultured RAW264.7 macrophages on pre-stretched and control PDMS membranes.
  • Assessed macrophage behavior including morphology, F-actin intensity, adhesion, migration, phagocytosis, reactive oxygen species generation, proliferation, and inflammatory marker expression (TNFα, IL-1β, Arg1, IL-10).

Main Results:

  • Pre-stretching altered PDMS surface topography (reduced step height and roughness) without significantly changing elastic modulus or water contact angle.
  • Macrophages on pre-stretched surfaces showed increased cell and nuclear spreading, enhanced adhesion, and improved migratory activity.
  • Pre-stretched surfaces promoted phagocytosis and reactive oxygen species generation, and induced a pro-inflammatory phenotype (elevated TNFα and IL-1β expression and secretion).

Conclusions:

  • Mechanically preconditioned PDMS surfaces act as instructive interfaces that modulate macrophage behavior, including morphology, motility, and inflammatory polarization.
  • Substrate mechanics represent an underappreciated regulator of macrophage function and inflammatory responses.
  • This study provides a valuable platform for biomaterials research, disease modeling, and drug screening in the context of macrophage mechanoimmunology.