Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

Published on: November 18, 2016

A Single-Cell Bioprinting Method to Reconstruct Native Cellular Microenvironments with Subcellular Resolution.

Luiz Bertassoni^1,2,3, Haylie Helms¹, Kody Oyama^1,3,4,5

¹Knight Cancer Precision Biofabrication Hub, Knight Cancer Institute, Oregon Health and Science University, Portland, OR 97201, USA.

Research Square

|June 12, 2026

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Novel Non-Valved Glaucoma Drainage Device with Reduced Inner Lumen Diameter: 6-Month Safety and Efficacy Outcomes.

Clinical ophthalmology (Auckland, N.Z.)·2026

Same author

Cell fusion reprograms tumor cells and promotes RUNX1-mediated invasion and dissemination in colorectal cancer.

bioRxiv : the preprint server for biology·2026

Same author

In clinically hyperthyroid cats, is I-131 treatment superior to thyroidectomy in normalising serum T4 level?

Veterinary evidence·2026

Same author

Plasma periaxin is a biomarker of peripheral nerve demyelination.

Brain : a journal of neurology·2025

Same author

Engineering neuronal networks in granular microgels to innervate bioprinted cancer organoids on-a-chip.

Lab on a chip·2025

Same author

Permissive central tolerance plus defective peripheral checkpoints license pathogenic memory B cells in CASPR2-antibody encephalitis.

Science advances·2025

Same journal

HIV Transmission Dynamics in Greater Mexico City are Shaped by Dense Spatial Mixing.

Research square·2026

Same journal

A UCP1-IRES-Cre Knock-In Mouse Enables Specific Brown Adipocyte Targeting Without CNS Off-Target Expression.

Research square·2026

Same journal

Precision RNAi for Fibrodysplasia Ossificans Progressiva: a combinatorial, unimolecular, allele selective approach.

Research square·2026

Same journal

Perceptions of end-of-life care quality among bereaved closest contacts of community-dwelling older Australians: a cross-sectional survey of the ASPREE cohort.

Research square·2026

Same journal

Heavy-chain immune repertoire sequencing enables language-model prediction of antigen-specific antibodies.

Research square·2026

Same journal

25+ Years of TRPV4: From Discovery to Translational Horizons.

Research square·2026

See all related articles

This summary is machine-generated.

Researchers developed a novel single-cell bioprinting platform to precisely reconstruct cellular microenvironments. This technology enables detailed studies of cell interactions and tissue organization, advancing our understanding of complex biological systems.

Area of Science:

Biotechnology
Tissue Engineering
Cell Biology

Background:

Cellular spatial organization is crucial for tissue development and function.
Existing experimental platforms lack the precision to reconstruct complex cellular arrangements.
Systematic interrogation of cell-cell interactions in native-like microenvironments is needed.

Purpose of the Study:

To introduce a novel single-cell bioprinting platform for programmable reconstruction of cellular microenvironments.
To enable precise control over single-cell placement and intercellular spacing.
To facilitate the study of spatial organization's impact on tissue function.

Main Methods:

Development of a single-cell bioprinting platform with subcellular spatial control.

More Related Videos

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Published on: September 22, 2015

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

Related Experiment Videos

Last Updated: Jun 13, 2026

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

Published on: November 18, 2016

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Published on: September 22, 2015

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

Demonstration of multiplexed deposition of up to eight cell types.

Reconstruction of biopsy-derived cellular organization with high accuracy (99% single-cell placement).

Main Results:

Achieved intercellular spacing as low as 1.3 µm.
Successfully reconstructed native tissue cellular organization from biopsies.
Demonstrated manipulation of cellular arrangement to study spatial transcriptional programs, cell signaling, and migration.

Conclusions:

The single-cell bioprinting platform enables programmable reconstruction of cellular microenvironments with high precision.
This technology allows for causal interrogation of spatially defined cell-cell interactions.
The platform offers a generalizable framework for studying complex tissue microenvironments at single-cell resolution.