Intraoral fluoride retention after using experimental toothpaste containing titanium tetrafluoride and chitosan

Area of Science:

• Dental research
• Materials science
• Biochemistry

Background:

• Fluoride toothpaste is crucial for caries prevention.
• Titanium tetrafluoride (TiF₄) and Chitosan (Ch) offer potential benefits for intraoral fluoride retention.
• Novel formulations are needed to enhance and prolong fluoride availability in the oral cavity.

Purpose of the Study:

• To evaluate the intraoral fluoride retention of a novel Titanium tetrafluoride (TiF₄)/Chitosan (Ch) toothpaste.
• To compare the fluoride retention of TiF₄/Ch toothpaste against TiF₄ alone, a positive control (Elmex® Caries Protection), and a placebo.
• To assess fluoride levels in stimulated saliva and dental biofilm at 1 and 12 hours post-brushing.

Main Methods:

• A double-blind, crossover clinical study involving 20 participants.
• Participants used four types of toothpaste: TiF₄/Ch, TiF₄, Elmex® Caries Protection, and Placebo.
• Stimulated saliva and biofilm samples were collected 1 and 12 hours after brushing, with fluoride concentrations measured using specific electrodes.

Main Results:

• TiF₄/Ch toothpaste significantly increased fluoride levels in saliva and biofilm 1 hour after brushing, comparable to TiF₄ and Elmex®.
• Elevated fluoride levels persisted in solid and total biofilm at 12 hours post-brushing.
• Fluoride levels in saliva and biofilm fluid returned to baseline by 12 hours.

Conclusions:

• TiF₄/Ch toothpaste effectively maintains fluoride retention in stimulated saliva and biofilm.
• The fluoride retention in biofilm persisted for 12 hours, indicating prolonged availability.
• TiF₄/Ch toothpaste shows promise as an alternative for sustained intraoral fluoride reservoirs.