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Related Experiment Video

Updated: Jun 16, 2026

The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability

The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability

Published on: April 28, 2022

CITF1 fine-tunes FIT activity to regulate Fe uptake.

Yuerong Cai^1,2, Rihua Lei^1,2, Junhui Zhao^1,2

¹State Key Laboratory of Plant Diversity and Specialty Crops, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Yunnan 650223, China.

|June 13, 2026

Summary

This summary is machine-generated.

The CU-DEFICIENCY INDUCED TRANSCRIPTION FACTOR 1 (CITF1) regulates plant iron uptake by modulating the central regulator FER-LIKE IRON DEFICIENCY INDUCED TRANSCRIPTION FACTOR (FIT). CITF1 fine-tunes FIT activity through multiple mechanisms, ensuring iron homeostasis.

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Area of Science:

Plant Biology
Molecular Biology
Biochemistry

Background:

Iron (Fe) is crucial for plant growth, requiring sophisticated uptake systems under deficiency.
FER-LIKE IRON DEFICIENCY INDUCED TRANSCRIPTION FACTOR (FIT) is a key regulator of plant iron uptake.
Maintaining iron homeostasis involves intricate modulation of FIT activity.

Purpose of the Study:

To investigate the role of CU-DEFICIENCY INDUCED TRANSCRIPTION FACTOR 1 (CITF1) in regulating plant iron uptake.
To elucidate the mechanisms by which CITF1 modulates FIT activity.

Main Methods:

Functional and genetic analyses were employed.
Mechanistic investigations were conducted to understand protein interactions and localization.
Gene expression analysis related to iron uptake.

Main Results:

CITF1 regulates iron uptake in a FIT-dependent manner.
CITF1 interferes with BTSL1/2 interaction with FIT.
CITF1 sequesters FIT protein from the nucleus to the cytoplasm.
CITF1 forms a dimer with FIT, inhibiting its transcriptional activity.

Conclusions:

CITF1 acts as a novel regulator of plant iron uptake by fine-tuning FIT activity.
CITF1 employs multiple mechanisms to control FIT function, contributing to iron homeostasis.
This study provides new insights into the complex regulatory network of iron uptake in plants.