Related Experiment Video
Updated: Jun 16, 2026
Fentanyl Analog Screening using LC-TIMS-TOF MS/MS
Published on: November 8, 2024
Comparative stability assessment of novel LSD analogs in dry blood spots and blood using UHPLC-MS/MS: implications
Kaja Tusiewicz1, Paweł Szpot2, Marcin Zawadzki3
1Department of Forensic Medicine, Wroclaw Medical University, 4 J. Mikulicza-Radeckiego Street, Wroclaw, 50-345, Poland. kaja.tusiewicz@student.umw.edu.pl.
Purpose:
New LSD analogs are a newly emerging and basically under-researched group of new psychoactive substances (NPS). The emergence of a completely new group of NPS make it difficult to monitor intoxications with these compounds. Moreover, forensic toxicological evaluation often requires comprehensive information on changes in the concentration of NPS in biological specimens over time following their collection.
Methods:
In this study, 12 LSD analogs were analyzed to determine their stability in whole blood and dry blood spots (DBS). The samples were stored at room temperature (DBS), 4 °C (DBS and whole blood), and - 20 °C (whole blood). Analyses were performed using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry. The stability study lasted 365 days, monitoring both the degradation rate of analytes and formation of degradation products in vitro. Moreover, the effect of chemical structure on analyte stability was assessed.
Results:
Results proved that the stability of analytes depended on both storage conditions and their chemical structure. Majority of compounds showed better long-term stability in DBS samples. Furthermore, N-deacylation of ALD-52, 1P-LSD, 1B-LSD, 1 V-LSD, 1cP-MiPLA, and 1P-MiPLA was observed, which was more pronounced for LSD derivatives than for MiPLA derivatives.
Conclusions:
Studies indicate a strong correlation between the stability of LSD analogs and both their chemical structure and storage conditions. DBS cards frequently provide equaled or enhanced stability compared to whole blood, which makes it advisable to collect biological material in both forms to help maximize analytical options in forensic and toxicological investigations.
