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Updated: Jun 16, 2026
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Combining Imaging and Electrophysiology to Visualize and Record Spreading Depolarizations in Mice
Published on: October 4, 2024
Spatiotemporal dynamics of direct cortical response depression during spreading depolarizations
Daria Vinokurova1, Bulat Mingazov1, Gulshat Zakirova1
1Laboratory of Neurobiology, Kazan Federal University, Kazan, Russia.
Summary
Spreading depolarizations (SDs) suppress cortical activity, including direct cortical responses (DCRs). The pattern of DCR suppression reveals the direction of SD propagation, aiding in detection and assessment.
Area of Science:
- Neuroscience
- Computational Neuroscience
Background:
- Spreading depolarizations (SDs) are waves of intense neuronal activity followed by suppression.
- Direct cortical responses (DCRs) are sensitive indicators of cortical function that are depressed during SDs.
Purpose of the Study:
- To investigate the spatiotemporal dynamics of DCR depression during SDs.
- To determine if DCR depression patterns can predict SD propagation direction.
Main Methods:
- Induction of SDs using high-potassium or pinprick stimuli in anesthetized rats.
- Simultaneous recording of cortical activity using ECoG arrays and intracortical silicon probes.
- Analysis of DCR onset and offset times in relation to DC shifts during SDs.
Main Results:
- SDs consistently suppressed DCRs, spontaneous activity, and sensory-evoked responses.
- The spatiotemporal pattern of DCR depression was dependent on SD propagation direction relative to stimulation and recording sites.
- DCR depression patterns provided reliable estimates of SD propagation direction.
Conclusions:
- The spatiotemporal organization of DCR depression during SDs offers insights into SD propagation dynamics.
- An algorithm analyzing DCR depression patterns can aid in SD detection and direction assessment during multisite ECoG recordings.
