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Related Experiment Video

Updated: Jun 16, 2026

Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers
11:14

Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers

Published on: April 17, 2018

Influence of Solvent Lipid Extraction on Tissue-Specific Carbon Isotopes.

J Groß1,2,3, B Fry4, J Eggebo1

  • 1Southern Ocean Persistent Organic Pollutants Program, Centre for Planetary Health and Food Security Griffith University Nathan Queensland Australia.

Ecology and Evolution
|June 15, 2026
PubMed
Summary

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Interpretation of southern hemisphere humpback whale diet via stable isotopes; implications of tissue-specific analysis.

PloS one·2023
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Assessing the effects of lipid extraction and lipid correction on stable isotope values (δ<sup>13</sup> C and δ<sup>15</sup> N) of blubber and skin from southern hemisphere humpback whales.

Rapid communications in mass spectrometry : RCM·2021
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<sup>15</sup>N natural abundances and N use by tundra plants.

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Stable isotope and trace metal compositions of Australian prawns as a guide to authenticity and wholesomeness.

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Detoxification enzyme activities (CYP1A1 and GST) in the skin of humpback whales as a function of organochlorine burdens and migration status.

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"Do it yourself" reference materials for δ13C determinations by isotope ratio mass spectrometry.

Analytical and bioanalytical chemistry·2013

Solvent lipid extraction for stable isotope analysis in cetaceans does not affect carbon-13 or nitrogen-15 values in pure proteins. However, multiple extractions are needed for accurate whale blubber analysis.

Area of Science:

  • Marine biology
  • Stable isotope ecology
  • Biogeochemistry

Background:

  • Cetacean tissues like blubber are lipid-rich, requiring lipid normalization for accurate stable isotope analysis (δ¹³C and δ¹⁵N).
  • Solvent effects on δ¹⁵N are debated, but solvent impacts on δ¹³C during lipid extraction remain understudied.

Purpose of the Study:

  • To investigate the effects of solvent lipid extraction on δ¹³C and δ¹⁵N in pure proteins and humpback whale blubber.
  • To assess the efficiency of multiple lipid extraction cycles.

Main Methods:

  • Lipid extraction using dichloromethane and methanol on four pure proteins and humpback whale blubber.
  • Analysis of δ¹³C and δ¹⁵N values before and after multiple extraction cycles.
  • Monitoring C:N ratios to assess protein content.
Keywords:
Southern Oceanblubberbovinebulk stable isotopesdietary evaluationhumpback whalessharksolvent extraction

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Last Updated: Jun 16, 2026

Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers
11:14

Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers

Published on: April 17, 2018

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Main Results:

  • Dichloromethane and methanol did not significantly alter δ¹³C and δ¹⁵N in pure proteins (within analytical error).
  • Humpback whale blubber δ¹³C significantly increased with each extraction cycle.
  • Consistent protein-like C:N ratios in blubber were achieved only after three lipid extractions.

Conclusions:

  • Solvent lipid extraction does not introduce significant analytical artifacts for δ¹³C beyond lipid removal.
  • Gentle lipid extraction methods are insufficient; multiple cycles are crucial for reliable isotopic data from lipid-rich tissues like cetacean blubber.