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Updated: Jun 16, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
Characterization of BAS-1, a novel class A β-lactamase encoded in Bacteroides thetaiotaomicron
María Aznar Fernández1,2, María José Gómez Gómez1,2, José Miguel Cisneros1,2,3,4
1Clinical Unit of Infectious Diseases, Microbiology and Parasitology, University Hospital Virgen del Rocío, Seville, Spain.
Abstract:
We characterized a novel class A β-lactamase (BAS-1) in a pan-β-lactam-resistant Bacteroides thetaiotaomicron clinical isolate. Whole-genome sequencing identified blaBAS-1 within a mobile genetic element associated with IS4351. Cloning, susceptibility testing, β-lactam inactivation method, and half-maximal inhibitory concentration assay confirmed resistance to penicillins, cephalosporins, monobactams, and reduced susceptibility to the β-lactamase inhibitors. To date, no class A β-lactamase from Bacteroides has been shown to display such a resistant profile, challenging the assumed reliability of β-lactam/β-lactamase inhibitor combinations.
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