An integrated rotational paper-based ELISA platform for multiplex inflammatory biomarker analysis in whole blood with...

In the context of global health, commercial centrifuges are effective precision separation tools. However, their high cost, large size, and dependence on a stable power supply hinder improvements in medical standards and limit the adoption of point-of-care testing (POCT) technology in resource-limited settings. Here, we have designed a novel and convenient detection platform that combines the function of a DIY centrifuge for whole blood pretreatment with a rotational paper-based analytical device for a multiplex sandwich-type enzyme-linked immunosorbent assay (ELISA). The platform is used to detect the inflammatory biomarkers C-reactive protein (CRP) and procalcitonin (PCT). The rotational paper-based analytical device demonstrates excellent analytical performance for CRP and PCT, with detection limits of 659 pg mL^-1 for CRP and 15.04 pg mL^-1 for PCT, respectively. We believe that this reliable technical platform offers a promising solution for disease diagnosis in resource-limited settings or remote regions.