A sodium carbonate-soluble polysaccharide from Amomum tsao-ko attenuates colitis via ZO-1-mediated barrier restoration and transcriptomics-implicated mucin glycosylation
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Updated: Jun 19, 2026

Establishment of Coloproctitis Cancer Model in Mice and Evaluation of Therapeutic Effect of Chinese Medicine
Published on: October 13, 2023
Xinyuan Yu1, Ting Li1, Sijia Li1
1State Key Laboratory of Bioactive Molecules and Druggability Assessment, Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou, 510632, China.
Abstract:
In this study, four polysaccharides (water-soluble, ATPW; 0.05 M Na2CO3-soluble ATP-SC; 0.1 M NaOH soluble ATP-SA1; and 0.5 M NaOH soluble ATP-SA2) were sequentially extracted from Amomum tsao-ko. Their physiochemical properties, monosaccharide compositions, molecular weights, and structural features were characterized. Their bioactivities were compared in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice, focusing on repairing the mucus barrier. Among them, ATP-SC displayed the strongest protective effects. Transcriptomics indicated that ATP-SC modulated mucin-type O-glycan biosynthesis, tight junction, and inflammatory signaling pathways associated with its mechanism of action. Furthermore, in vivo validation revealed that ATP-SC enhanced intestinal barrier integrity, while decreasing immune cells infiltration. In vitro, ATP-SC suppressed inflammation in macrophages, and restored tight junction integrity in damaged intestinal epithelial cells. Collectively, this study identifies ATP-SC as the key bioactive polysaccharide from A. tsao-ko, acting through mucin glycosylation-driven mucus barrier restoration, highlighting its potential as a functional food-derived protective agents for UC.
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