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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Updated: Jun 20, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

CRISPR-Based Programmable RNA-Responsive Protein Materials.

Yingjie Xu1,2,3, Songzi Kou2, Xinyu Huang1

  • 1Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.

ACS Macro Letters
|June 18, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed programmable RNA-responsive protein materials using CRISPR-Cas7-11 for biosensing and therapeutics. These smart materials detect specific RNA sequences, triggering payload release for applications like viral detection and biofilm degradation.

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

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Area of Science:

  • Biomaterials Science
  • Molecular Biology
  • Synthetic Biology

Background:

  • RNA biology and biotechnologies are rapidly advancing.
  • Smart materials with RNA responsiveness are crucial for biosensing, diagnostics, and therapeutics.
  • CRISPR-Cas7-11 is an RNA-guided protease with potential for programmable material systems.

Purpose of the Study:

  • To develop a programmable RNA-responsive protein material system.
  • To leverage CRISPR-Cas7-11 for sequence-specific RNA detection and payload release.
  • To demonstrate applications in viral RNA sensing and bacterial detection.

Main Methods:

  • Immobilized CRISPR-Cas7-11 protease complexes and cleavable payload proteins onto protein scaffolds using SpyTag/SpyCatcher chemistry.
  • Developed two material platforms: synthetic spider-silk fibers and protein hydrogels.
  • Utilized sequence-specific RNA detection to trigger payload release (e.g., GFP, PslG).

Main Results:

  • Successfully created programmable RNA-responsive protein materials.
  • Demonstrated sequence-specific RNA detection capabilities.
  • Showcased controlled release of payloads, including a biofilm-degrading enzyme.
  • Validated applications in viral RNA sensing and *Pseudomonas aeruginosa* detection.

Conclusions:

  • The developed protein material system offers a versatile platform for RNA-responsive applications.
  • CRISPR-Cas7-11 integration enables precise control over material function based on RNA targets.
  • This technology holds promise for advanced biosensing, diagnostics, and targeted therapeutics, including antimicrobial strategies.