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Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule01:10

Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule

In the AX proton spin system, proton A can sense the two spin states of a coupled proton X, resulting in a doublet NMR signal with two peaks of equal (1:1) intensity. When proton A is coupled to two equivalent protons (AX2 spin system), the spin states of each X can be aligned with or against the external field, creating three possible scenarios. This results in a 1:2:1  triplet signal, where the central peak corresponds to the chemical shift of A and is twice as large or intense as the others.
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...

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Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
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STRIP: A processing method to improve peakshapes in spectra acquired from multidimensional phase-modulated NMR

Christopher John Bauer1

  • 1United Kingdom of Great Britain and Northern Ireland.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|June 18, 2026
PubMed
Summary

A new processing method called STRIP can reduce phase-twist shapes in two-dimensional NMR spectra. This method alleviates problems caused by dispersive components in phase-modulated data.

Keywords:
Homonuclear J-spectroscopyPhase-twistPure shiftSTRIPℬ transform

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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Spectroscopic Data Processing

Background:

  • Two-dimensional NMR spectroscopy can produce phase-modulated data with problematic phase-twist peak shapes.
  • These phase-twist peaks result from a mixture of absorption and dispersion components, which are difficult to correct using standard phase correction techniques.

Purpose of the Study:

  • To introduce a novel processing method, STRIP, designed to mitigate the issue of phase-twist peaks in 2D NMR spectra.
  • To reduce the impact of dispersive components in phase-modulated NMR data.

Main Methods:

  • Development and application of the STRIP (Signal-to-True-In-Phase) processing algorithm.
  • Utilizing a newly introduced B transform within the STRIP algorithm.
  • The algorithm is designed to be compact and easy to understand.

Main Results:

  • The STRIP method effectively reduces the size of double dispersion components in phase-twist peaks.
  • While not achieving a perfectly pure absorption mode spectrum, the reduction in dispersive components significantly alleviates associated problems.
  • The method is applicable to spectra where all relevant absorption peaks share the same sign.

Conclusions:

  • The STRIP algorithm offers a practical solution for reducing phase-twist artifacts in 2D NMR.
  • This processing method enhances the quality of spectra derived from phase-modulated data.
  • STRIP provides a valuable tool for NMR spectroscopists dealing with dispersive peak components.