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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

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Optimization of multisite reactions in complex compartmentalized media.

T V Mendes^1,2, T Guérin¹

¹University of Bordeaux, CNRS, Laboratoire Ondes et Matière d'Aquitaine, /, F-33400 Talence, France.

Physical Review. E

|June 19, 2026

Summary

Adding a crowded compartment can accelerate complex reactions, especially for weakly reactive targets. Optimal compartment size minimizes reaction time, even outperforming systems without compartments.

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Optimization of Radiochemical Reactions using Droplet Arrays

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Area of Science:

Chemical Kinetics
Statistical Physics
Complex Systems

Background:

Transport and geometry influence reaction kinetics in complex media.
Subdiffusion in fractal media affects reactant encounter rates.
Dual-site reactions require two activation events within a time window.

Purpose of the Study:

Investigate the effect of a fractal compartment on reaction kinetics.
Determine if a compartment can accelerate reaction times.
Identify optimal conditions for reaction acceleration.

Main Methods:

Modeling random walker behavior in a diffusing medium with an obstructed fractal compartment.
Analytical predictions for reaction time.
Validation through simulations on a cubic lattice at the percolation threshold.

Main Results:

Reaction time can be minimized with an optimal compartment size.
Compartments can accelerate reactions compared to systems without them, particularly for weakly reactive targets.
Crowded compartments, despite slowing local motion, can enhance overall reaction kinetics.

Conclusions:

Compartmentalization in complex media can be a strategy to accelerate reaction kinetics.
The geometry and transport properties of the medium play crucial roles in reaction dynamics.
Understanding these factors is key for designing efficient chemical processes.