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Published on: July 12, 2019
Establishment of a novel rat model of chronic subdural hematoma: Recapitulating human pathological features through
Qiuyu Lu1,2, Renkun Zhang1,3,2, Yuhao Tan1,2
1Neurovascular Center, Changhai Hospital, Naval Medical University, China.
Objectives:
This study aimed to develop a novel rat model of Chronic subdural hematoma (CSDH) that better recapitulates human pathophysiological mechanisms, enhancing clinical relevance for translational research.
Materials And Methods:
The CSDH model was established using a triple-component system comprising allogeneic venous blood, cerebrospinal fluid (CSF), and cerebral microvascular endothelial cells (bEnd.3). Stereotaxic surgery involved subdural injection of the mixture (200 µL) via a microsyringe pump, followed by two additional injections at 72-hour intervals. Subsequently, systematic validation of the model was conducted. Data were analyzed using GraphPad Prism 9. All procedures adhered to ARRIVE guidelines and ethical approval.
Results:
The CSF-blood co-culture system generated mechanically stable, heterotypically remodeled clots with ultrastructural features mirroring human CSDH membranes. The optimized CSDH model exhibited crescent-shaped subdural hematomas on MRI, temporal concordance in VEGF/Ang-2/MMP-9 expression peaks (day 14), and progressive neurological recovery, closely recapitulating clinical-pathological progression.
Conclusion:
This study establishes a reproducible CSDH model combining progressive subdural injection and stereotaxic localization to recapitulate chronic hematoma dynamics, validated through quantitative morphological, inflammatory, and functional profiling, thereby providing a clinically faithful platform for mechanistic and therapeutic investigation.
