Protocol for ex vivo physicochemical assessment of photothermally preconditioned platelet-rich plasma

Photothermal biomodulation is used to precondition platelet-rich plasma, but standardized laboratory workflows for monitoring associated physicochemical changes remain limited. This article describes a paired ex vivo protocol for assessing selected physicochemical parameters of platelet-rich plasma before and after photothermal preconditioning.•Platelet-rich plasma is prepared from donor blood, pooled per donor, and divided into paired control and photothermally preconditioned aliquots.•The protocol enables time-course monitoring of density/turbidity, pH, and temperature from baseline to 60 min.•Application in samples from three healthy donors showed that the workflow can detect small time-dependent differences between paired aliquots.