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Related Experiment Video

Updated: Jun 20, 2026

CRISPR-Cas-mediated Multianalyte Synthetic Urine Biomarker Test for Portable Diagnostics
04:33

CRISPR-Cas-mediated Multianalyte Synthetic Urine Biomarker Test for Portable Diagnostics

Published on: December 8, 2023

Rapid identification directly from urine using QUICKprep kit.

M Sousa-Pinheiro1,2, I Martins-Oliveira3, D Abreu3

  • 1RISE-Health, Faculty of Medicine, University of Porto, Porto, Portugal.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|June 19, 2026
PubMed
Summary

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Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

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This summary is machine-generated.

This study validates the QUICKprep® kit for rapid urinary tract infection diagnosis. It enables faster identification of bacteria from urine samples, improving treatment decisions.

Area of Science:

  • Clinical microbiology
  • Diagnostic technology

Background:

  • Urinary tract infections (UTIs) are prevalent, frequently caused by Escherichia coli.
  • Current culture-based diagnostics take 48 hours, leading to empirical and potentially ineffective antibiotic treatments.

Purpose of the Study:

  • To validate the QUICKprep® (FASTinov®) kit for rapid microbial identification in urine samples.
  • To assess the kit's efficiency in preparing samples for MALDI-TOF-MS analysis.

Main Methods:

  • The QUICKprep® kit was used to concentrate viable microbial cells and remove debris from urine samples.
  • Identifications were performed using Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS).
  • A total of 281 urine samples, pre-screened as positive, were analyzed.
Keywords:
Direct identificationMALDI-TofQUICKprepUrinary tract infection

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Cell-Free DNA Integrity Analysis in Urine Samples
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Published on: January 5, 2017

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Last Updated: Jun 20, 2026

CRISPR-Cas-mediated Multianalyte Synthetic Urine Biomarker Test for Portable Diagnostics
04:33

CRISPR-Cas-mediated Multianalyte Synthetic Urine Biomarker Test for Portable Diagnostics

Published on: December 8, 2023

Cell-Free DNA Integrity Analysis in Urine Samples
07:58

Cell-Free DNA Integrity Analysis in Urine Samples

Published on: January 5, 2017

Main Results:

  • The QUICKprep® kit processed samples in under five minutes.
  • Inoculated samples showed complete concordance with traditional culture methods.
  • MALDI-TOF-MS identification using QUICKprep®-prepared samples took approximately 15 minutes.
  • 13 out of 155 clinical samples were not identified by MALDI-TOF-MS.

Conclusions:

  • The QUICKprep® kit facilitates rapid and accurate bacterial identification directly from urine.
  • This method offers a significant reduction in diagnostic time compared to conventional culture.
  • Faster diagnosis has the potential to guide antimicrobial therapy and improve patient outcomes.