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UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for electronic transitions. As a result...

UV–Vis Spectroscopy of Conjugated Systems

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π&VeryThinSpace;→&VeryThinSpace;π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...

UV–Vis Spectroscopy: Molecular Electronic Transitions

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

UV–Vis Spectrum

UV–Vis Spectrum

When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...

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Related Experiment Video

Updated: Jun 21, 2026

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds

Published on: October 18, 2018

Multiscale Ultraviolet/Visible Absorption Spectroelectrochemistry in Parallel Configuration.

Maria Huidobro¹, Martin Perez-Estebanez¹, Aranzazu Heras¹

¹Department of Chemistry, Universidad de Burgos, Pza. Misael Bañuelos s/n, E-09001 Burgos, Spain.

Analytical Chemistry

|June 19, 2026

Summary

This summary is machine-generated.

This study introduces multiscale UV/vis-SEC, a new spectroelectrochemistry technique. It uses a dual path length cell for reliable optical measurements, improving UV/vis absorption data quality.

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UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

Published on: October 25, 2021

Area of Science:

Analytical Chemistry
Electrochemistry
Spectroscopy

Background:

High-quality UV/vis absorption measurements are vital for reliable spectroelectrochemistry (UV/vis-SEC) data.
Accurate initial light intensity (I₀) is essential for calculating absorbance (A = -log(I/I₀)).
Low I₀ or I values can distort UV/vis spectra, especially for species with high absorption coefficients.

Purpose of the Study:

To develop an improved UV/vis-SEC technique for enhanced data reliability.
To address challenges associated with low light intensity measurements in UV/vis-SEC.
To accurately characterize the evolution of electroactive species during electrode processes.

Main Methods:

Development of a novel multiscale UV/vis-SEC technique.
Utilization of a dual path length cell for simultaneous optical measurements.
Application of the technique to study electrode processes.

Main Results:

The multiscale UV/vis-SEC technique enables simultaneous optical measurements at different scales.
The dual path length cell improves the reliability of UV/vis spectra.
The method accurately describes the evolution of reactants and products in electrode processes.

Conclusions:

Multiscale UV/vis-SEC offers a reliable approach for spectroelectrochemistry.
The technique enhances the quality of UV/vis absorption measurements.
This method is valuable for studying complex electrode processes with high absorption species.