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Hehua Quan¹, Jiayi He¹, Feilu Wang¹

¹Department of Anesthesiology and Perioperative Medicine, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University; Key Laboratory of Pediatric Anesthesiology (Wenzhou Medical University), Ministry of Education; Key Laboratory of Precision Anesthesiology of Zhejiang Province, Wenzhou Medical University, Wenzhou, 325027, Zhejiang Province, China.

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Summary

This summary is machine-generated.

Fibroblast Growth Factor 9 (FGF9) promotes Leydig cell (LC) proliferation and testosterone production by increasing H3K4me3 histone modifications. This study clarifies FGF9

Keywords:

FGF9 H3K4me3 modification SLCs development testosterone(T)

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Area of Science:

Reproductive Biology
Endocrinology
Epigenetics

Background:

Fibroblast Growth Factor 9 (FGF9) is crucial for testis development.
Histone modifications, particularly H3K4me3, are implicated in steroidogenesis.
The precise role of FGF9 and histone modifications in Leydig cell (LC) differentiation is not fully understood.

Purpose of the Study:

To investigate the effect of FGF9 on Leydig cell (LC) differentiation and testosterone synthesis.
To explore the involvement of histone modifications, specifically H3K4me3, in FGF9-mediated effects.
To elucidate the regulatory mechanisms of FGF9 in the male reproductive system.

Main Methods:

Adult male Sprague-Dawley rats were treated with ethane dimethane sulfonate (EDS) to deplete LCs, followed by intratesticular FGF9 injection.
Quantitative PCR (qPCR) and Western blot were used to assess gene and protein expression related to testosterone synthesis.
Chromatin immunoprecipitation sequencing (ChIP-seq) identified H3K4me3 binding regions.
In vitro tubule culture was performed to study FGF9 effects on stem LCs.

Main Results:

FGF9 treatment increased serum testosterone levels, LC number, and expression of key steroidogenic genes.
FGF9 significantly elevated H3K4me3 levels and reduced H3K9me3 levels.
FGF9 promoted H3K4me3 enrichment on the promoter regions of steroidogenic genes (Scarb1, Stard1, Srd5a1).
In vitro, FGF9 enhanced testosterone production and stem LC proliferation.

Conclusions:

FGF9 stimulates Leydig cell (LC) proliferation and differentiation.
FGF9 enhances testosterone production through epigenetic regulation via H3K4me3 modification.
These findings highlight FGF9 as a key regulator in testicular function and steroidogenesis.