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Related Experiment Video

Updated: Jun 23, 2026

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized ¹³C NMR

Published on: July 8, 2025

Automated para-Hydrogen Hyperpolarization for Mixture Analysis Using ¹H and ¹³C Benchtop NMR Detection.

Daniel A Taylor¹, James McCall¹, Fraser Hill-Casey¹

¹Department of Chemistry, University of York, Heslington, York, North Yorkshire YO10 5DD, U.K.

Analytical Chemistry

|June 20, 2026

Summary

Signal amplification by reversible exchange (SABRE) hyperpolarization enhances benchtop NMR for mixture analysis. Automation provides repeatable hyperpolarization, enabling sensitive and resolved spectra for complex samples.

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Published on: November 2, 2018

Area of Science:

Analytical Chemistry
Spectroscopy
Nuclear Magnetic Resonance (NMR)

Background:

Benchtop NMR spectroscopy offers accessible mixture analysis but faces limitations in sensitivity and signal resolution.
Standard high-field NMR provides better sensitivity and resolution but is less accessible.
Signal amplification by reversible exchange (SABRE) hyperpolarization is a technique to improve NMR sensitivity.

Purpose of the Study:

To develop an automated workflow for SABRE hyperpolarization to enhance benchtop NMR for complex mixture analysis.
To demonstrate the repeatability and integration capabilities of the automated SABRE hyperpolarization system.
To achieve fully resolved NMR spectra for mixtures with improved signal-to-noise ratios.

Main Methods:

Automated SABRE hyperpolarization workflow for NMR experiments.
Integration of hyperpolarization into standard NMR pulse sequences.
Acquisition of 1D ¹³C{¹H} and 2D ¹³C-¹H HETCOR and ¹H-¹H COSY spectra.

Main Results:

Highly repeatable hyperpolarization with 2.5% relative standard deviation in signal enhancement over 100 experiments.
Repolarization times of approximately 13 seconds.
Fully resolved ¹³C{¹H} benchtop NMR spectra with signal-to-noise ratios up to 40 for a 3 mM mixture.
Resolved overlapped ¹H NMR peaks using SABRE-enhanced 2D ¹³C-¹H HETCOR.
Analysis of a 750 μM mixture using SABRE-enhanced 2D ¹H-¹H COSY in a single scan.

Conclusions:

Automated SABRE hyperpolarization significantly enhances benchtop NMR capabilities for sensitive and resolved mixture analysis.
The developed workflow is repeatable, efficient, and integrable with various NMR experiments.
This approach enables detailed analysis of complex mixtures at lower concentrations, expanding the utility of benchtop NMR.