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Updated: Jun 23, 2026
08:32
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
Published on: May 23, 2025
CRISPR systems in cancer therapeutics and diagnostics
Mohsen Almakrami1, Zubair Ahmed2, Ghadah M Alali3
1Ministry of Heath Regional Lab and central Blood Bank Najran 66271, Saudi Arabia. mmalmakrami@moh.gov.sa.
Cellular and Molecular Biology (Noisy-Le-Grand, France)
|June 20, 2026
Summary
CRISPR gene editing offers precise cancer diagnostics and therapies by targeting oncogenes and improving immunotherapy. This technology also aids in identifying drug resistance and detecting cancer biomarkers for personalized treatment strategies.
Area of Science:
- Biotechnology
- Genomics
- Oncology
Background:
- Cancer remains a leading global cause of death, necessitating advanced diagnostic and therapeutic methods.
- The CRISPR-Cas9 system provides a powerful tool for precise genome editing in cancer research.
Purpose of the Study:
- To review CRISPR-based applications in cancer therapeutics and diagnostics.
- To explore CRISPR's potential in targeting oncogenes, tumor suppressors, and immunotherapy.
- To examine CRISPR's role in identifying drug resistance and detecting cancer biomarkers.
Main Methods:
- Review of CRISPR/Cas9 mechanism and its application in cancer biology.
- Analysis of CRISPR-based therapeutic strategies, including CAR-T cell engineering.
- Examination of CRISPR diagnostic approaches for detecting cancer DNA, vesicles, proteins, and microRNAs.
Main Results:
- CRISPR enables targeting oncogenes, restoring tumor suppressor function, and enhancing cancer immunotherapy.
- CRISPR screening identifies genes linked to drug resistance.
- CRISPR technologies demonstrate high sensitivity and specificity in detecting cancer biomarkers.
Conclusions:
- CRISPR systems are expanding their role in cancer diagnosis and therapy.
- CRISPR holds significant potential for developing personalized and effective cancer management strategies.
- Addressing challenges like off-target effects and delivery is crucial for clinical translation.
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