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Updated: Jun 23, 2026

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
Published on: December 16, 2015
A high-quality chromosome-level genome assembly of Ficus carica cultivar 'Green Peel'
Hantang Huang1, Tengyue Zhang1, Pinqi Sun1
1College of Horticulture, China Agricultural University, Beijing, 100193, China.
Abstract:
Fig (Ficus carica L.) is an early-domesticated fruit crop of high cultural and economic value. However, high-quality genome resources for evolutionary and genetic diversity studies remain limited. Here we report a high-quality, chromosome-level genome assembly for fig cv. 'Green Peel' generated with Oxford Nanopore long reads and Hi-C data, and polished with Illumina reads. The final assembly is 306.83 Mb and comprises 13 pseudo-chromosomes, with a contig N50 of 5.77 Mb. Read mapping supported the assembly, with mapping rate and genome coverage exceeding 99.5%, and BUSCO completeness reaching 98.1%. Annotation predicted 27,036 protein-coding genes with 97.3% BUSCO completeness, together with 1,303 transcription factors and 2,080 non-coding RNAs. Comparative analyses with Ficus pumila characterized gene family expansion and duplication modes and identified 131.43 Mb of structural variation. This dataset provides a valuable resource for trait mapping, genome-assisted breeding, and future fig pan-genome studies.
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