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Related Experiment Video

Updated: Jun 23, 2026

Ultrasound Localization Microscopy for Super-Resolution Mapping of the Rodent Brain Microvasculature
10:36

Ultrasound Localization Microscopy for Super-Resolution Mapping of the Rodent Brain Microvasculature

Published on: November 14, 2025

Functional Ultrasound Localization Microscopy on Freely Moving Rats.

Yike Wang, Bohan Zhao, Matthew R Lowerison

    Research Square
    |June 22, 2026
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a new system for functional ultrasound localization microscopy (fULM) enabling brain-wide, single-vessel resolution imaging in freely moving rats. This breakthrough allows detailed study of brain hemodynamics during natural behaviors.

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    Related Experiment Videos

    Last Updated: Jun 23, 2026

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    A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents
    08:02

    A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents

    Published on: July 16, 2020

    Area of Science:

    • Neuroscience
    • Biomedical Engineering
    • Medical Imaging

    Background:

    • Brain-wide functional neuroimaging at single-vessel resolution in freely moving animals is challenging.
    • Functional ultrasound localization microscopy (fULM) provides high-resolution hemodynamic imaging but is typically limited to head-fixed preparations.

    Purpose of the Study:

    • To develop and validate a platform for fULM in freely moving rats.
    • To enable high-resolution, brain-wide hemodynamic imaging under naturalistic behavioral conditions.

    Main Methods:

    • Integration of a magnetic probe-clamping interface, a pulley-based tether, and chronic jugular venous access.
    • Development of a motion-correction processing pipeline for freely moving ULM data.
    • Utilized visual stimulation and pharmacological agents (SR-17018) to probe brain responses.

    Main Results:

    • The platform reliably captured functional hemodynamic responses and enabled super-resolved vascular reconstruction in deep brain regions.
    • Freely moving fULM resolved stimulus-evoked, vessel-specific changes in diameter and flow velocity.
    • Observed transient, region-dependent cerebrovascular responses to an opioid agonist, with significant flow-velocity changes.

    Conclusions:

    • Freely moving fULM is established as a practical platform for whole-brain microvascular imaging in naturalistic behavioral settings.
    • This technology reveals microvascular heterogeneity not detectable by conventional methods.
    • Enables advanced research into brain function and pharmacology in behaving animals.