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STAR Protocols question-and-answer series: CRISPR
1STAR Protocols, Cell Press.
STAR Protocols
|June 22, 2026
Summary
CRISPR technology revolutionizes genome engineering for precise gene editing. This guide offers expert advice on CRISPR strategies and troubleshooting for biological and clinical research success.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- CRISPR technology offers precise genome engineering capabilities.
- Successful application in research relies on robust protocols and troubleshooting.
- Expert insights are crucial for advancing CRISPR techniques.
Purpose of the Study:
- To provide expert advice on CRISPR technologies.
- To address common questions regarding genome-editing strategies.
- To offer troubleshooting approaches for CRISPR applications.
Main Methods:
- Compilation of expert advice from STAR Protocols authors.
- Discussion of common questions and challenges in CRISPR research.
- Sharing of practical troubleshooting strategies.
Main Results:
- Key insights into effective CRISPR implementation.
- Guidance on optimizing genome-editing strategies.
- Solutions for common CRISPR-related experimental issues.
Conclusions:
- Expert guidance enhances the successful application of CRISPR technology.
- Addressing common challenges improves research reproducibility.
- This resource aids researchers in biological and clinical settings.
Related Concept Videos
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
01:59CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
01:23The Antiviral System of Bacteria and Archaea: CRISPR
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
02:53CRISPR and crRNAs
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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01:28CRISPR/Cas9 Genome Editing
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
02:31Homologous Recombination
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...