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Related Experiment Video

Updated: Jun 24, 2026

A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures
08:55

A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures

Published on: November 8, 2024

Focus Stacking Protocol for High-Resolution Insect Photography.

Nathanaël Maury1, Somchit Sudavanh1, Nita Soun2

  • 1Toxo Inc.

Journal of Visualized Experiments : Jove
|June 22, 2026
PubMed
Summary
This summary is machine-generated.

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This study introduces a low-cost, standardized photographic focus stacking protocol for high-resolution insect imaging. The method enhances accessibility for biodiversity digitization and taxonomy, producing artifact-free images efficiently.

Area of Science:

  • Entomology
  • Imaging Science
  • Digital Photography

Background:

  • High-resolution imaging of insect specimens is crucial for taxonomy and biodiversity studies.
  • Existing methods can be costly and lack standardized workflows, limiting accessibility.

Purpose of the Study:

  • To present a reproducible, low-cost protocol for acquiring extended depth-of-field images of insects.
  • To establish a standardized workflow for insect specimen imaging.

Main Methods:

  • Utilized a modular digital imaging system with a high-resolution mirrorless camera and microscope objectives.
  • Employed photographic focus stacking with synchronized strobe illumination and sub-micron focus increments.
  • Developed a standardized workflow encompassing equipment assembly, calibration, image acquisition, and post-processing.

Related Experiment Videos

Last Updated: Jun 24, 2026

A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures
08:55

A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures

Published on: November 8, 2024

Main Results:

  • Achieved high-resolution images with pixel scales from 0.76 µm to 0.19 µm.
  • Produced artifact-free composite images through precise focus stacking.
  • The protocol captures and processes approximately 20 images per week under laboratory conditions.

Conclusions:

  • The developed protocol offers an accessible, low-cost alternative to commercial systems for insect imaging.
  • Standardized workflow ensures reproducibility, supporting large-scale biodiversity digitization and taxonomic efforts.
  • Enables production of color-calibrated identification plates for research and outreach.