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Related Experiment Video

Updated: Jun 24, 2026

A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures

A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures

Published on: November 8, 2024

Focus Stacking Protocol for High-Resolution Insect Photography.

Nathanaël Maury¹, Somchit Sudavanh¹, Nita Soun²

¹Toxo Inc.

Journal of Visualized Experiments : Jove

|June 22, 2026

Summary

This summary is machine-generated.

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Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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This study introduces a low-cost, standardized photographic focus stacking protocol for high-resolution insect imaging. The method enhances accessibility for biodiversity digitization and taxonomy, producing artifact-free images efficiently.

Area of Science:

Entomology
Imaging Science
Digital Photography

Background:

High-resolution imaging of insect specimens is crucial for taxonomy and biodiversity studies.
Existing methods can be costly and lack standardized workflows, limiting accessibility.

Purpose of the Study:

To present a reproducible, low-cost protocol for acquiring extended depth-of-field images of insects.
To establish a standardized workflow for insect specimen imaging.

Main Methods:

Utilized a modular digital imaging system with a high-resolution mirrorless camera and microscope objectives.
Employed photographic focus stacking with synchronized strobe illumination and sub-micron focus increments.
Developed a standardized workflow encompassing equipment assembly, calibration, image acquisition, and post-processing.

Related Experiment Videos

Last Updated: Jun 24, 2026

A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures

A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures

Published on: November 8, 2024

Main Results:

Achieved high-resolution images with pixel scales from 0.76 µm to 0.19 µm.
Produced artifact-free composite images through precise focus stacking.
The protocol captures and processes approximately 20 images per week under laboratory conditions.

Conclusions:

The developed protocol offers an accessible, low-cost alternative to commercial systems for insect imaging.
Standardized workflow ensures reproducibility, supporting large-scale biodiversity digitization and taxonomic efforts.
Enables production of color-calibrated identification plates for research and outreach.