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Updated: Jun 24, 2026

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
11:30

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Published on: June 2, 2022

Association between soluble Klotho levels and calcification propensity T50 in patients with hemodialysis: a

Akio Nakashima1, Kazuhiko Kato2, Arisa Kobayashi2

  • 1Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo, Japan. a-nakashima@jikei.ac.jp.

Scientific Reports
|June 22, 2026
PubMed

Soluble Klotho is an anti-aging hormone implicated in vascular calcification. The calcification propensity score (T50) is a method used to evaluate the calcification propensity. However, no study has evaluated the association between soluble Klotho and T50. Therefore, this study aimed to investigate the association between soluble Klotho and T50 in patients undergoing hemodialysis. This cross-sectional study recruited patients undergoing hemodialysis. We measured T50 using the turbidimetric method and soluble Klotho using an enzyme-linked immunosorbent assay. Multiple regression analysis was used to examine the association between soluble Klotho and T50. In total, 199 patients were included in this study. Patients' mean age was 65.2 ± 10.2 years, and the median soluble Klotho concentration was 408 (321-525) pg/mL. The serum T50 value was 78 (63-104) minutes. The correlation coefficient between T50 and soluble Klotho was 0.39 (p < 0.001). Multivariate analysis revealed that the T50 value was significantly longer when comparing the group with the lowest and highest Klotho concentrations (β-coefficient = 21.3 [95% confidence interval: 4.5-38.2, p < 0.013]). The analysis of hemodialysis patients revealed for the first time that Klotho is associated with the T50 time. Soluble Klotho may be involved in vascular calcification.

Keywords:
HemodialysisKlothoT50Vascular calcification

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