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Updated: Jun 24, 2026

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
Published on: October 6, 2020
Smart double-screening system of propagating male-sterile lines for maize hybrid seed production
Ruidan Liu1, Shengxi Hou1, Hui Guo1
1State Key Laboratory of Maize Bio-breeding, Key Laboratory of Maize Biology and Genetic Breeding, National Maize Improvement Center, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
Abstract:
The Seed and Seedling Marker Method double-screening system for maize hybrid seed production integrates Ms45-mediated fertility restoration, Mn1/Sh1-RNAi-based seed selection, and Lc-based seedling selection. It thus enables efficient, high-purity propagation of male-sterile lines by combining fertility maintenance with sequential visual selection at the seed and seedling stages.

