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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

The Antiviral System of Bacteria and Archaea: CRISPR

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Related Experiment Video

Updated: Jun 25, 2026

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

An Entropy-Driven Autocatalysis-Regulated Signal-On CRISPR/Cas12a Biosensor Supported by DNA Triangular Prism

Li Yang¹, Ya Zhou¹, Huimin Li¹

¹College of Chemistry and Materials Science, Sichuan Normal University, Chengdu 610066, China.

Analytical Chemistry

|June 23, 2026

Summary

This study introduces a novel biosensor combining entropy-driven autocatalysis and CRISPR/Cas12a for ultrasensitive biomarker detection. The system achieves high sensitivity and specificity, showing promise for early hepatocellular carcinoma diagnosis.

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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

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Published on: December 23, 2022

Area of Science:

Biomedical Engineering
Molecular Diagnostics
Biochemistry

Background:

Detecting low-abundance biomarkers in complex samples is difficult due to amplification/background trade-offs.
Conventional biosensing methods often suffer from high background interference.

Purpose of the Study:

To develop a synergistic amplification strategy for ultrasensitive and specific biomarker detection.
To circumvent high background interference in biosensing.
To create a versatile biosensing framework for early disease diagnosis.

Main Methods:

Integrated entropy-driven autocatalysis (EDAC) with a signal-on CRISPR/Cas12a assay and a DNA triangular prism (DTP) interface.
Utilized EDAC for exponential signal amplification and inhibition of CRISPR/Cas12a.
Employed DTP as a scaffold for ordered nucleic acid assembly and electrochemical readout.

Main Results:

Achieved ultrasensitive detection of hepatocellular carcinoma biomarkers (AFP and miR-122) with detection limits of 11.37 fg/mL and 18.13 aM.
Demonstrated high specificity and minimal background interference.
Showed excellent agreement with ELISA in clinical serum samples (AUC=1.00).

Conclusions:

The synergistic amplification strategy offers a robust platform for ultrasensitive biosensing.
The developed biosensor shows significant potential for early hepatocellular carcinoma diagnosis and clinical translation.