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Integrated physiological, transcriptomic and metabolomic analyses shed light on the complex mechanisms underlying low
Mengmeng Wen1, Yue Li1, Hongshan Lang1
1College of Horticulture, Yantai Technology Center of Characteristic Plant Gene Editing & Germplasm Innovation, Ludong University, Yantai, Shandong Province, 264025, China.
Abstract:
Actinidia arguta is an emerging fruit crop in northern China, characterized by its strong tolerance to low temperature (LT). Investigating the LT stress response of A. arguta is essential to elucidate the intricate resistance mechanisms in kiwifruit. For this purpose, A. arguta seedlings were subjected to 4 ◦C LT stress for 0, 12, 24 and 48 h. Physiological analyses revealed that LT stress induced a significant increase in the activities of key antioxidant enzymes, concurrent with the elevated levels of malonaldehyde (MDA) and proline. Transcriptome profiling identified 2297 genes with consistent expression changes across all treatment groups, among which the plant hormone signaling pathway was the most significantly enriched. Furthermore, weighted gene co-expression network analysis (WGCNA) pinpointed 15 hub genes positively correlated with LT resistance in A. arguta. In addition, the integrative analysis of transcriptome and metabolome revealed that the starch and sucrose metabolism pathway exhibited the most consistent changes, spanning from gene expression to metabolite accumulation. Specifically, LT stress induced changes in the levels of two important metabolites sucrose and UDP-glucose and the expressions of 32 genes encoding carbohydrate metabolic enzymes under LT stress. In conclusion, this study sheds light on the intricate mechanisms of LT resistance in kiwifruit, and provides gene resources for the molecular breeding of LT resistant kiwifruit varieties.
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