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Published on: May 14, 2020
Molecular insights into Trx system genes identified the role of miR6427-5p-PagCDSP32 module in poplar under drought
Yuzhang Yang1, Yunfei Zhu1, Zeqi Wang1
1State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Biotechnology, Beijing Forestry University, BeijingChina.
Abstract:
Thioredoxins (TRXs), a class of ubiquitous oxidoreductases, are essential for thiol redox regulation in plants. So far, the Trx system has been established in herbaceous plants. However, studies of the Trx system and its posttranscriptional regulation mediated by miRNAs in woody plants are scarce, and especially the functions of the regulatory mode in poplar responding to drought remain unclear. In this investigation, antioxidant enzyme activities and osmotic adjustment responses suggested that the chloroplast-specific antioxidants were indispensable for regulating the drought tolerance of poplar. The Trx system genes were comprehensively identified and classified, and the miRNA-mediated posttranscriptional regulation of Trx system genes was investigated in poplar, revealing the high number of atypical TRXs and their special functions. PagCDSP32, an atypical TRX located in the chloroplast, was identified as a drought-tolerant candidate. Transgenic poplars overexpressing PagCDSP32 exhibited stronger reactive oxygen species (ROS) scavenging ability and drought tolerance, while those with suppressed PagCDSP32 expression showed the opposite. Transcriptomic analysis indicated that differentially expressed genes affected by PagCDSP32 were mainly enriched in maintaining cell homeostasis. PagCDSP32 could interact with 2 peroxiredoxins, and inhibition of miR6427-5p expression increased the ROS scavenging ability of poplar under drought stress by liberating the cleavage of PagCDSP32. Intriguingly, the expression of the miR6427-5p-PagCDSP32 module determined the drought tolerance of poplar varieties. Overall, a potential module regulating poplar drought tolerance was proposed, which would provide a valuable resource of Trx system genes in poplars and facilitate future efforts to decipher the role of TRXs in regulating plant tolerance to abiotic stresses.
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