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Related Experiment Video

Updated: Jun 25, 2026

In Vitro Model for Studying Differentiation and Changes of Multi-Omics on Murine Airway Epithelial Cells Stimulated with Cigarette Smoke Extract
09:07

In Vitro Model for Studying Differentiation and Changes of Multi-Omics on Murine Airway Epithelial Cells Stimulated with Cigarette Smoke Extract

Published on: July 12, 2024

Single-Cell Transcriptomic Landscape of Smoking-Related Periodontitis.

Huining Wang1,2, Pengxiao Hu1, Jiayu Wu3

  • 1Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Oral Diseases
|June 24, 2026
PubMed
Summary

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This summary is machine-generated.

Smoking alters gingival epithelial cells, causing senescence and chemokine release. These cells may recruit regulatory T cells (Tregs), influencing inflammation in smoking-related periodontitis.

Area of Science:

  • Oral Biology
  • Immunology
  • Genomics

Background:

  • Periodontitis is a common inflammatory disease affecting gums.
  • Smoking is a major risk factor exacerbating periodontitis severity.
  • Understanding the cellular and molecular mechanisms of smoking-related periodontitis (SPD) is crucial.

Purpose of the Study:

  • To investigate the cellular composition and molecular microenvironment of SPD.
  • To identify key cell types and molecular pathways involved in SPD pathogenesis.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) on periodontal tissues from healthy, periodontitis, and SPD patients.
  • Immunofluorescence validation of key gene expression and cell subtypes.
  • Analysis of cellular senescence, chemokine secretion, and cell-cell interactions.
Keywords:
gingivamucosal immunityperiodontitissingle‐cell RNA sequencingsmoking

Related Experiment Videos

Last Updated: Jun 25, 2026

In Vitro Model for Studying Differentiation and Changes of Multi-Omics on Murine Airway Epithelial Cells Stimulated with Cigarette Smoke Extract
09:07

In Vitro Model for Studying Differentiation and Changes of Multi-Omics on Murine Airway Epithelial Cells Stimulated with Cigarette Smoke Extract

Published on: July 12, 2024

Main Results:

  • Gingival epithelial cells were significantly impacted by smoking, showing senescence and increased CXCL16 secretion in SPD.
  • A specific subset of CXCR6+ regulatory T cells (Tregs) was identified in SPD.
  • CXCR6+ Tregs may be recruited by epithelial cells via the CXCL16-CXCR6 axis and influence Th17 cell differentiation and inflammation.

Conclusions:

  • Gingival epithelial cells in SPD undergo senescence and release chemokines.
  • Senescent epithelial cells may recruit CXCR6+ Tregs, potentially modulating Th17 cell responses.
  • These findings offer insights into the molecular basis of SPD.