Phyto-engineered CuO nanoparticles from gum Eucalyptus camaldulensis: a GC-MS, molecular docking, and bioactivity
Farwa Nasir1, Sofia Javed1, Muhammad Irfan1
1Department of Biochemistry and Biotechnology, University of Gujrat, Gujrat, Pakistan.
Aims:
To profile the phytochemicals of gum Eucalyptus camaldulensis and evaluate its potential for the synthesis of CuO nanoparticles (NPs).
Methods:
Gum constituents were identified by GC-MS. The CuO NPs were synthesised and characterised using UV-Vis, DLS, TEM, FTIR, EDX, and XRD. Antibacterial, photocatalytic, and molecular docking analyses were performed.
Results:
GC-MS revealed 21 phytochemicals. NPs showed UV-Vis absorption at 260-270 nm, an average hydrodynamic diameter of 135.2 ± 3 nm, and a TEM size of 83 ± 2 nm. FTIR confirmed phytochemical involvement in NPs formation, while XRD verified a monoclinic crystalline structure. NPs exhibited antibacterial activity against E. coli (25 ± 1 mm) and B. subtilis (16.5 ± 1 mm) and degraded crystal violet dye. Oleic acid showed favourable docking scores with bacterial targets.
Conclusion:
Gum E. camaldulensis is an effective phytochemical resource for producing bioactive CuO NPs with antibacterial and photocatalytic potential.
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