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Updated: Jun 26, 2026
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
Published on: August 3, 2015
Protocol for enhancing production of human PSC-derived cardiomyocytes and endothelial cells with placental growth
Liang Liu1, Phuong Thao Nguyen1, Jie Liu1
1Department of Regenerative Medicine, Hamamatsu University School of Medicine, Hamamatsu City, Shizuoka 431-3192, Japan.
Abstract:
Placental growth factor (PLGF) drives cardiomyogenesis and vasculogenesis during cardiogenesis. Here, we present a protocol for enhancing production of human pluripotent stem cell (hPSC)-derived cardiomyocytes (CMs) and endothelial cells (ECs). We describe steps for CM-/EC-directed hPSC differentiation with PLGF. Our approaches can achieve production of hPSC-CMs/-ECs by 2- to 2.5-fold compared with those in traditionally established protocols, through promoting proliferation and differentiation of cardiac and vascular progenitors. For complete details on the use and execution of this protocol, please refer to Witman et al.1.
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