Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Concentration and depth-resolved analysis of transition metals in atmospheric particles: A combined approach using X-ray fluorescence and synchrotron radiation single-particle imaging.

Journal of environmental sciences (China)·2026

Same author

High-throughput in situ single particle X-ray imaging of dehydrating viral capsids.

Light, science & applications·2026

Same author

High-Precision Laser Time-Frequency Synchronization in Space Based on an Improved Kalman Filtering Method.

Sensors (Basel, Switzerland)·2026

Same author

Single-Particle X-ray Scattering Reveals a High Local Supersaturation of Precursors as the Origin of CoO Assembly Formation.

The journal of physical chemistry letters·2026

Same author

Redirection and reshaping of intense extreme-ultraviolet radiation.

Science advances·2026

Same author

Molecular magnetic X-ray scattering with ultrashort X-ray free-electron lasers: from spin-orbit dynamics to Berry phase detection.

Physical chemistry chemical physics : PCCP·2026

Same journal

Plasmonic nanocomposite helices for weather-adaptive LiDAR function.

Nature communications·2026

Same journal

Multidirectional strain-insensitive stretchable RF electronics.

Nature communications·2026

Same journal

In-scanner thoughts contribute to resting-state functional connectivity.

Nature communications·2026

Same journal

Metal-center electron affinity modulates multicolor electrochromism in 2D conjugated metal-organic frameworks.

Nature communications·2026

Same journal

Hyperbranched dielectric polymer networks exhibiting giant energy storage density at 250 °C.

Nature communications·2026

Same journal

3D nanoprinting of metals by spatiotemporally confined hot electrons via multiple-electron excitations in nanocrystals.

Nature communications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 26, 2026

$Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene$

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

Dichography: two-frame ultrafast imaging from a single diffraction pattern.

Linos Hecht¹, Andre Al Haddad², Björn Bastian³

¹Laboratory for Solid State Physics, ETH Zurich, Zurich, Switzerland.

Nature Communications

|June 24, 2026

Summary

This summary is machine-generated.

Researchers developed Dichography, a new method using dual X-ray pulses to capture two snapshots of nanomaterials. This technique achieves 20 nm resolution, revealing structural changes over time and enabling ultrafast imaging.

More Related Videos

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

Related Experiment Videos

Last Updated: Jun 26, 2026

$Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene$

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

Area of Science:

* Ultrafast X-ray science
* Nanomaterial imaging
* Coherent diffractive imaging

Background:

* X-ray Free Electron Lasers (XFELs) offer ultrabright and ultrashort pulses for probing matter dynamics.
* Capturing time-resolved structural dynamics of nanomaterials requires high spatial and temporal resolution.
* Overlapping diffraction signals from multiple pulses complicate data analysis.

Purpose of the Study:

* To introduce and validate Dichography, a novel method for separating and reconstructing images from time-delayed X-ray pulses.
* To achieve high-resolution, time-resolved imaging of nanomaterials using dual-color X-ray pulses.
* To investigate the timescale of structural damage in nanomaterials under intense X-ray illumination.

Main Methods:

* Experimental setup utilizing pairs of time-delayed, dual-color X-ray pulses from an X-ray Free Electron Laser.
* Development of the Dichography algorithm to computationally separate overlapping diffraction patterns.
* Reconstruction of two distinct images from single diffraction events for structural analysis.

Main Results:

* Successful reconstruction of two time-delayed images of xenon-doped helium nanodroplets with 20 nm spatial resolution.
* Observation of structural consistency in nanodroplets up to 750 fs delay, indicating damage occurs at longer timescales.
* Validation of the method by imaging silver nanoparticles with high fidelity.

Conclusions:

* Dichography effectively separates diffraction signals, enabling time-resolved imaging with XFELs.
* The study provides insights into the ultrafast structural dynamics and damage thresholds of nanomaterials.
* This method opens new avenues for time-resolved studies in physics, chemistry, and materials science.