Related Experiment Video
Updated: Jun 26, 2026
06:56
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
Published on: March 31, 2017
Transcriptome sequence resource for the hazelnut powdery mildew pathogen Erysiphe corylacearum.
1Department of Genetics and Bioengineering, Giresun University, Giresun, 28200, Türkiye. ulkubaykal@yahoo.com.
Scientific Data
|June 24, 2026
Summary
This study provides the first comprehensive transcriptomic dataset for Erysiphe corylacearum, the fungus causing hazelnut powdery mildew. This resource aids in understanding the pathogen and developing better disease management strategies.
Area of Science:
- Plant Pathology
- Mycology
- Genomics
Background:
- Erysiphe corylacearum causes significant economic damage to hazelnut crops.
- Limited molecular resources exist for this obligate biotrophic pathogen.
- Understanding its biology is crucial for disease management.
Purpose of the Study:
- To generate the first comprehensive transcriptomic dataset for E. corylacearum.
- To provide molecular resources for studying hazelnut powdery mildew.
- To facilitate research into pathogen biology and host-pathogen interactions.
Main Methods:
- Illumina sequencing of mRNA from infected hazelnut leaves.
- Targeted epidermal peeling to enrich fungal material.
- De novo Trinity-based assembly and functional annotation.
Main Results:
- Generated over 66 million high-quality paired-end reads.
- Assembled 100,824 transcript sequences with 71% functional annotation.
- Identified sequences related to pathogenicity, sexual compatibility, and reproduction.
- Discovered over 29,000 intra-sample sequence variants.
Conclusions:
- The generated transcriptomic dataset is a valuable resource for E. corylacearum research.
- This resource will support the development of diagnostic assays and marker development.
- Facilitates future investigations into hazelnut powdery mildew biology and management.
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