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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: Jun 26, 2026

A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro
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SPR Sensing: From Biomolecular Interactions to Cell-Based Analysis.

Petia Genova-Kalou1, Evdokiya O Hikova2, Todor Kereziev3

  • 1National Center of Infectious and Parasitic Diseases, 44A Gen. Stoletov Blvd., 1233 Sofia, Bulgaria.

Biosensors
|June 25, 2026
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Summary

Surface Plasmon Resonance (SPR) techniques have advanced for detecting molecular interactions within cells. This review details the evolution of cell-based SPR, focusing on grating-coupled SPR for biological assays.

Keywords:
cell-based analysiscytotoxicitymorphologypharmacokineticsproliferationsurface plasmon resonance

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Area of Science:

  • Biophysics
  • Biotechnology

Background:

  • Surface Plasmon Resonance (SPR) is established for quantifying biomolecular interactions in vitro.
  • Cell-based SPR techniques have emerged over the last 20-25 years for in situ molecular interaction analysis.
  • Diverse biological research necessitates advanced SPR methods for detecting numerous parameters.

Purpose of the Study:

  • To chronologically review the development of cell-based SPR techniques.
  • To identify key factors driving the evolution of SPR methodologies.
  • To focus on grating-coupled SPR for cell assays and compare it with other SPR techniques.

Main Methods:

  • Review of chronological development of SPR techniques.
  • Focus on grating-coupled SPR (GC-SPR) for cell-based assays.
  • Comparative analysis of SPR techniques based on biological object studies.

Main Results:

  • Established SPR for in vitro biomolecular interaction quantification.
  • Emergence of cell-based SPR for physiological environment analysis.
  • Grating-coupled SPR shows specific capabilities for cell assays.

Conclusions:

  • Cell-based SPR offers precise detection of molecular interactions in vivo.
  • Grating-coupled SPR is a promising technique for cell-based biological studies.
  • Future SPR techniques and applications are predicted based on current trends.