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Related Experiment Video

Updated: Jun 26, 2026

Measuring Skeletal Muscle Thermogenesis in Mice and Rats
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Measuring Skeletal Muscle Thermogenesis in Mice and Rats

Published on: July 27, 2022

Nicotinamide Riboside Supplementation Protects Against Heat-Induced Skeletal Muscle Injury in Female Mice.

Yifan Chen1, Tianzheng Yu1,2

  • 1Department of Military and Emergency Medicine, F. Edward Hébert School of Medicine, Uniformed Services University, Bethesda, MD 20814, USA.

Muscles (Basel, Switzerland)
|June 25, 2026
PubMed
Summary

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Nicotinamide riboside (NR) supplementation protects female mice against heat-induced skeletal muscle injury by reducing inflammation and oxidative stress. This study highlights NR

Area of Science:

  • Muscle Physiology
  • Nutritional Biochemistry
  • Heat Stress Biology

Background:

  • Micronutrients are vital for muscle metabolic health.
  • Heat stress causes skeletal muscle injury, linked to NAD+ and Mg2+ depletion.
  • Nicotinamide riboside (NR) previously protected male mice against heat-induced muscle injury.

Purpose of the Study:

  • To investigate if NR supplementation prevents heat-induced skeletal muscle injury in female mice.
  • To determine the effects of NR on NAD+ and Mg2+ levels during heat stress in females.
  • To elucidate the underlying mechanisms of NR's protective effects.

Main Methods:

  • Female mice received daily oral NR or vehicle for 10 days.
  • Mice underwent sham or heat exposure experiments.
Keywords:
ATPgenderheat stresshyperthermianiacinskeletal muscle

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  • Muscle tissue was analyzed for NAD+ and Mg2+ levels, inflammation, oxidative stress, mitochondrial function, and apoptosis.

    • Main Results:

    • NR supplementation did not alter muscle NAD+ levels but prevented heat-induced muscle injury in female mice.
    • Heat exposure significantly decreased muscle Mg2+ levels.
    • NR treatment attenuated heat-induced inflammation, oxidative stress, mitochondrial impairment, and apoptosis.

    Conclusions:

    • NR demonstrates a preventive effect against skeletal muscle heat injury in female mice.
    • The protective mechanisms involve anti-inflammatory, antioxidative, mitochondrial protective, and anti-apoptotic actions.
    • These benefits occur independently of alterations in muscle NAD+ homeostasis.