Exploring neutral backbones in phosphorodiamidate morpholino oligomers: synthesis and biophysical properties
Arnab Das1, Debajyoti Kuilya1, Swrajit Nath Sharma1
1School of Applied and Interdisciplinary Sciences, Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700032, India. ocss5@iacs.res.in.
Abstract
None:
The synthesis of morpholino dimer chlorophosphoramidates and phosphoramidites has been successfully achieved and the method has been used for short oligomer synthesis. Neutral backbone chemistries like urea, carbamate, methylene and glycine linkages were introduced into a 12-mer PMO, followed by thermal melting and circular dichroism analysis. The findings provide a foundation for the development of next-generation antisense oligonucleotides.


