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Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
Association of Epithelial-to-Mesenchymal Transition Related Markers With Clinical Outcome in Non-Muscle Invasive and
R Singh1, Singh Up2, Minal Garg3
1Department of Biochemistry, University of Lucknow, Lucknow, 226007, India.
Cell Biochemistry and Biophysics
|June 25, 2026
Summary
Epithelial-to-mesenchymal transition (EMT) markers are clinically significant in bladder cancer. Altered EMT profiles predict survival outcomes and aid in risk stratification for non-muscle invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC) patients.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Epithelial-to-mesenchymal transition (EMT) plays a crucial role in cancer progression and metastasis.
- Understanding the clinical significance of EMT-related markers in bladder cancer is essential for improved patient management.
Purpose of the Study:
- To evaluate the clinical significance of epithelial-to-mesenchymal transition (EMT)-related markers in non-muscle invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC) patients.
- To determine the association of EMT marker expression with clinicohistopathological variables and survival outcomes.
Main Methods:
- Real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemical (IHC) staining were performed on 100 bladder tumor specimens (59 NMIBC, 41 MIBC).
- Expressions of epithelial markers, mesenchymal markers, and EMT-activating transcription factors (EMT-ATFs) were analyzed at mRNA and protein levels.
- Statistical associations between marker expression and clinicohistopathological variables, including survival, were investigated.
Main Results:
- Significant associations were found between EMT marker expression (e.g., E-cadherin, N-cadherin, Vimentin, Snail, Slug, Twist, Zeb-1) and various clinicohistopathological variables in both NMIBC and MIBC cohorts.
- Vimentin was identified as a predictor of shorter progression-free survival (PFS) and overall survival (OS) in MIBC patients.
- Altered EMT profiles independently predicted shorter recurrence-free survival (RFS) in NMIBC patients and shorter PFS and OS in MIBC patients.
Conclusions:
- EMT-related markers show significant associations with clinicohistopathological variables and survival outcomes in bladder cancer.
- EMT marker expression analysis can enhance risk stratification for NMIBC and MIBC patients.
- These findings underscore the importance of EMT markers in the clinical management of bladder cancer.
