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Updated: Jun 27, 2026
Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons
Published on: March 21, 2018
Protocol for simultaneous in vivo two-photon imaging and locomotion quantification during olfactory stimulation and
Qiantao Lv1, Yunming Gao2, Jing Ning2
1Zhejiang University, Hangzhou, Zhejiang 310058, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang 310024, China; Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310030, China; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310024, China.
Abstract:
To decipher the circuit and molecular mechanisms underlying neural information processing, it is essential to manipulate cellular targets while simultaneously recording neural activity and behavior during sensory stimulation. Here, we present a protocol for Drosophila that temporally synchronizes olfactory stimulation and pharmacological intervention with in vivo recording as well as locomotion measurement. We detail step-by-step workflows for head-fixed walking flies, including fly preparation, surgery, two-photon imaging, and locomotion detection, providing instructions for surgery station, odor stimulator, and perfusion system setup.
